Generator
Part:BBa_K797015:Design
Designed by: Tomohiro Nobeyama Group: iGEM12_Kyoto (2012-09-22)
FT generator with Histag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 513
Illegal BamHI site found at 277 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 84
Design Notes
6-Histag is inserted by PCR.
Source
FT gene is derived from Arabidopsis.
References
[1]Paula Teper-Bamnolker and Alon Samach1 (2005) "The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves